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cellasic onix b04a microfluidic plate  (Merck & Co)

 
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    Merck & Co cellasic onix b04a microfluidic plate
    Cellasic Onix B04a Microfluidic Plate, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cellasic+microfluidic+plate/onix2/bio_rxiv__2024__10__15__618391-212-8-12
    Average 90 stars, based on 1 article reviews
    cellasic onix b04a microfluidic plate - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Sterility:

    Article Title: Hyperpolarisation of Mitochondrial Membranes Is a Critical Component of the Antifungal Mechanism of the Plant Defensin, Ppdef1.
    Article Snippet: .. Spores were loaded with 1⁄2 PDB into the barrier traps and prewashed with sterile ultra-pure water, in a CellASIC microfluidic plate (Y04T, Merck) using the CellASIC ONIX microfluidic system. .. The plates were sealed with a breathable membrane (Sigma Aldrich) and incubated at 28 ◦C overnight (ON) for hyphal growth.

    Article Title: Hyperpolarisation of Mitochondrial Membranes Is a Critical Component of the Antifungal Mechanism of the Plant Defensin, Ppdef1
    Article Snippet: .. The cells in CDAA medium (1⁄2 Czapek Dox, 1⁄2 drop out mix complete amino acids, United States Biological, pH 5.0, Salem, USA) were then loaded into the wells of a CellASIC microfluidic plate (Y04C, Merck, Victoria, Australia), prewashed with sterile ultra-pure water using the CellASIC ONIX microfluidic system (Merck, Victoria, Australia), and examined using a Zeiss LSM 780 laser scanning confocal microscope (Carl Zeiss AG, Jena, Germany). .. To confirm FM4-64 vacuolar staining, the pre-labelled cells were observed using an LDC apo 40× water immersion/1.1 Korr M27 objective with FM4-64 excitation at 561 nm and fluorescence detection at 591 nm.

    Article Title: Hyperpolarisation of Mitochondrial Membranes Is a Critical Component of the Antifungal Mechanism of the Plant Defensin, Ppdef1
    Article Snippet: .. Spores were loaded with 1⁄2 PDB into the barrier traps and prewashed with sterile ultra-pure water, in a CellASIC microfluidic plate (Y04T, Merck) using the CellASIC ONIX microfluidic system. .. The plates were sealed with a breathable membrane (Sigma Aldrich) and incubated at 28 °C overnight (ON) for hyphal growth.

    Article Title: Hyperpolarisation of Mitochondrial Membranes Is a Critical Component of the Antifungal Mechanism of the Plant Defensin, Ppdef1.
    Article Snippet: Treatment of Vacuole-Stained Yeast Cells with FAM-Labelled Ppdef1 The vacuoles of S. cerevisiae cells were prestained with FM4-64 as described in Vida et al. [33]. .. The cells in CDAA medium (1⁄2 Czapek Dox, 1⁄2 drop out mix complete amino acids, United States Biological, pH 5.0, Salem, USA) were then loaded into the wells of a CellASIC microfluidic plate (Y04C, Merck, Victoria, Australia), prewashed with sterile ultra-pure water using the CellASIC ONIX microfluidic system (Merck, Victoria, Australia), and examined using a Zeiss LSM 780 laser scanning confocal microscope (Carl Zeiss AG, J. ..

    Microscopy:

    Article Title: Hyperpolarisation of Mitochondrial Membranes Is a Critical Component of the Antifungal Mechanism of the Plant Defensin, Ppdef1
    Article Snippet: .. The cells in CDAA medium (1⁄2 Czapek Dox, 1⁄2 drop out mix complete amino acids, United States Biological, pH 5.0, Salem, USA) were then loaded into the wells of a CellASIC microfluidic plate (Y04C, Merck, Victoria, Australia), prewashed with sterile ultra-pure water using the CellASIC ONIX microfluidic system (Merck, Victoria, Australia), and examined using a Zeiss LSM 780 laser scanning confocal microscope (Carl Zeiss AG, Jena, Germany). .. To confirm FM4-64 vacuolar staining, the pre-labelled cells were observed using an LDC apo 40× water immersion/1.1 Korr M27 objective with FM4-64 excitation at 561 nm and fluorescence detection at 591 nm.

    Article Title: Hyperpolarisation of Mitochondrial Membranes Is a Critical Component of the Antifungal Mechanism of the Plant Defensin, Ppdef1.
    Article Snippet: Treatment of Vacuole-Stained Yeast Cells with FAM-Labelled Ppdef1 The vacuoles of S. cerevisiae cells were prestained with FM4-64 as described in Vida et al. [33]. .. The cells in CDAA medium (1⁄2 Czapek Dox, 1⁄2 drop out mix complete amino acids, United States Biological, pH 5.0, Salem, USA) were then loaded into the wells of a CellASIC microfluidic plate (Y04C, Merck, Victoria, Australia), prewashed with sterile ultra-pure water using the CellASIC ONIX microfluidic system (Merck, Victoria, Australia), and examined using a Zeiss LSM 780 laser scanning confocal microscope (Carl Zeiss AG, J. ..



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    Image Search Results


    Journal: iScience

    Article Title: Epithelial uptake leads to fungal killing in vivo and is aberrant in COPD-derived epithelial cells

    doi: 10.1016/j.isci.2024.109939

    Figure Lengend Snippet:

    Article Snippet: CellASIC M04S-03 Microfluidics plates (Merck) were equilibrated and prepared as described by the manufacturers.

    Techniques: Purification, Recombinant, Clone Assay, Southern Blot, Plasmid Preparation, Software, Saline, Lysis, Imaging, Flow Cytometry, Microscopy

    (A) Schematic of S. cerevisiae life cycle. F0 = parental generation, F1 = filial generation 1 (B) Schematic microfluidic protocol to specifically trigger S. cerevisiae’s sexual life cycle by selecting against non-sporulated cells that can outcompete germinating spores. Nuclei represented as solid shapes inside the cells. (C-D) Representative time lapse micrographs of computationally aligned yeast cells undergoing (C) homothallic life cycle type A, in which sporulation is followed by ascus mating directly leading to two diploid cells without an intervening proliferative haploid state, or (D) Homothallic life cycle type B, in which sporulation is followed by partial ascus mating producing one diploid and two proliferative haploid cells which eventually diploidize through inbreeding.

    Journal: bioRxiv

    Article Title: Deep learning-driven imaging of cell division and cell growth across an entire eukaryotic life cycle

    doi: 10.1101/2024.04.25.591211

    Figure Lengend Snippet: (A) Schematic of S. cerevisiae life cycle. F0 = parental generation, F1 = filial generation 1 (B) Schematic microfluidic protocol to specifically trigger S. cerevisiae’s sexual life cycle by selecting against non-sporulated cells that can outcompete germinating spores. Nuclei represented as solid shapes inside the cells. (C-D) Representative time lapse micrographs of computationally aligned yeast cells undergoing (C) homothallic life cycle type A, in which sporulation is followed by ascus mating directly leading to two diploid cells without an intervening proliferative haploid state, or (D) Homothallic life cycle type B, in which sporulation is followed by partial ascus mating producing one diploid and two proliferative haploid cells which eventually diploidize through inbreeding.

    Article Snippet: The culture was spun down on a tabletop microfuge for 3 seconds to remove clumps, and 70 μl of the top layer were transferred to a microfluidics device with flow control (Y04C CellASIC plate with OniX controllers), pre-warmed at 25 °C.

    Techniques:

    (A) Schematic microfluidic induction of sequential S. cerevisiae sexual life cycles. Solid circles = nuclei; green = LiCH, orange = Whi5-mSC. (B) Representative scaled average size, Whi5-mSC or LiCHI nuclear concentration during the transition from cell birth into the first mitotic division aligned to the peak of Whi5 concentration during G1 (C-E) Boxplot comparison of the first mitotic division in three sexually reproducing generations (F0-F2) in terms of (C) cell size, (D) peak nuclear Whi5 levels, (E) total Whi5 cell concentration. (F-H) Boxplot comparison of the kinetics of meiotic divisions in three sexually reproducing generations (F0-F2) in terms of the duration between the time point of anaphase I and (F) pre-meiotic G1 exit, (G) prophase I exit, (H) meiotic exit. (I-K) Correlation between the time of anaphase I and pre-meiotic G1 exit in (I) F0, (J) F1, and (K) F2 generations. (L-N) Correlation between the time of anaphase I and cell size at anaphase I (L) F0, (M) F1, and (N) F2 generations.

    Journal: bioRxiv

    Article Title: Deep learning-driven imaging of cell division and cell growth across an entire eukaryotic life cycle

    doi: 10.1101/2024.04.25.591211

    Figure Lengend Snippet: (A) Schematic microfluidic induction of sequential S. cerevisiae sexual life cycles. Solid circles = nuclei; green = LiCH, orange = Whi5-mSC. (B) Representative scaled average size, Whi5-mSC or LiCHI nuclear concentration during the transition from cell birth into the first mitotic division aligned to the peak of Whi5 concentration during G1 (C-E) Boxplot comparison of the first mitotic division in three sexually reproducing generations (F0-F2) in terms of (C) cell size, (D) peak nuclear Whi5 levels, (E) total Whi5 cell concentration. (F-H) Boxplot comparison of the kinetics of meiotic divisions in three sexually reproducing generations (F0-F2) in terms of the duration between the time point of anaphase I and (F) pre-meiotic G1 exit, (G) prophase I exit, (H) meiotic exit. (I-K) Correlation between the time of anaphase I and pre-meiotic G1 exit in (I) F0, (J) F1, and (K) F2 generations. (L-N) Correlation between the time of anaphase I and cell size at anaphase I (L) F0, (M) F1, and (N) F2 generations.

    Article Snippet: The culture was spun down on a tabletop microfuge for 3 seconds to remove clumps, and 70 μl of the top layer were transferred to a microfluidics device with flow control (Y04C CellASIC plate with OniX controllers), pre-warmed at 25 °C.

    Techniques: Concentration Assay, Comparison